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15:28, 29 October 2024 JWTay talk contribs created page Python (Created page with " == Installing Python using Anaconda/Miniconda == '''Anaconda''' is a software package for Python that offers a number of functionalities including package management (download and install Python packages from various web sources), and a virtual environment manager. The latter is a highly recommended practice as it allows you to define ''environments'', which can each have a different Python version and installed packages. Anaconda comes with a number of GUI-based tools,...") Tag: Visual edit
15:20, 29 October 2024 JWTay talk contribs created page Bioimage analysis tools (Created page with "This page lists a number of commonly used bioimage analysis tools. Clicking on the links will take you to instructions on how to install and use the software. == GUI-based tools == GUI-based tools utilize a graphical interface for image analysis. These tools typically use manual adjustments by the user to determine parameters, such as the threshold intensity. These applications are likely the easiest to use for new users, but there is usually less flexibility in the way...") Tag: Visual edit
20:37, 15 September 2023 JWTay talk contribs created page File:Image pixelization.png
20:37, 15 September 2023 JWTay talk contribs uploaded File:Image pixelization.png
20:33, 15 September 2023 JWTay talk contribs created page File:How camera pixels digitize light.png
20:33, 15 September 2023 JWTay talk contribs uploaded File:How camera pixels digitize light.png
20:31, 15 September 2023 JWTay talk contribs created page File:Microscope imaging.png
20:31, 15 September 2023 JWTay talk contribs uploaded File:Microscope imaging.png
20:21, 15 September 2023 JWTay talk contribs created page MediaWiki:Citizen-footer-tagline (Created blank page)
20:20, 15 September 2023 JWTay talk contribs created page MediaWiki:Citizen-footer-desc (Created blank page)
19:40, 15 September 2023 JWTay talk contribs created page Image (Created page with "An image is a visual representation of the biological system under study. Typically, these images are digital and viewed on a computer. == How images are formed == Images are formed by focusing light from the sample on to a detector, such as a camera. For this section, we will focus on how 2D images are formed on a camera. A simplified diagram of the optical path from the sample to a camera is shown in Fig. 1. Light from the sample passes through the optical train of a...") Tag: Visual edit
17:41, 13 September 2023 JWTay talk contribs created page Image registration (Created page with "'''Image registration''' is the process of spatially aligning images in a dataset. Registration is often used to correct for drift or motion within an imaging dataset. Registration can also be used to align images taken from different viewpoints or by different equipment (e.g., aligning EM images with optical images). === Strategies to correct image drift === Drift in an image can be caused by different issues, including thermal changes (which causes expansion/contracti...") Tag: Visual edit
18:45, 12 September 2023 JWTay talk contribs created page Light (Created page with "Light is electromagnetic radiation that can be detected by the human eye. Light is typically defined by its intensity (brightness), and wavelength (color). == Wave theory == In the wave theory, light is described as a propagating wave of energy, similar to an ocean wave. * Amplitude - The total energy carried by the wave is amplitude * Wavelength - The distance propagated between troughs or peaks") Tag: Visual edit
18:35, 12 September 2023 JWTay talk contribs created page Fluorescence microscopy (Created page with "thumb|328x328px|Schematic of a digital fluorescence microscope. Light from a laser passes through an excitation laser and is reflected by a dichroic mirror. The light then excites fluorophores in the sample, which emit light. The emitted fluorescence passes through both the dichroic mirror and emission filter to form an image on a camera. '''Fluorescence microscopy''' is an imaging tool which uses a high intensity light source to exci...") Tag: Visual edit
18:25, 12 September 2023 JWTay talk contribs created page File:Fluorescence microscope.svg
18:25, 12 September 2023 JWTay talk contribs uploaded File:Fluorescence microscope.svg
19:01, 11 September 2023 JWTay talk contribs protected Main Page [Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite) (High traffic page) (hist)
18:51, 11 September 2023 JWTay talk contribs created page MediaWiki:Common.css (Created page with "CSS placed here will be applied to all skins: body.page-Main_Page li#ca-talk { display: none !important; }")
16:01, 11 September 2023 JWTay talk contribs created page File:Otsu's method - Figure 3.svg
16:01, 11 September 2023 JWTay talk contribs uploaded File:Otsu's method - Figure 3.svg
16:01, 11 September 2023 JWTay talk contribs created page File:Otsu's method - Figure 2.svg
16:01, 11 September 2023 JWTay talk contribs uploaded File:Otsu's method - Figure 2.svg
16:01, 11 September 2023 JWTay talk contribs created page File:Otsu's method - Figure 1.svg
16:01, 11 September 2023 JWTay talk contribs uploaded File:Otsu's method - Figure 1.svg
16:00, 11 September 2023 JWTay talk contribs created page Otsu's method (Created page with "'''Otsu's method''' is an image processing algorithm commonly used to compute a threshold level to generate a mask of objects within an image. The original algorithm<ref>N. Otsu, "A Threshold Selection Method from Gray-Level Histograms," in ''IEEE Transactions on Systems, Man, and Cybernetics'', vol. 9, no. 1, pp. 62-66, Jan. 1979, doi: 10.1109/TSMC.1979.4310076.</ref> calculates a global threshold - a single threshold level is calculated that divides the...") Tag: Visual edit: Switched
15:40, 11 September 2023 MediaWiki default talk contribs created page Main Page